Voyager DP-263-S Uživatelská příručka Strana 137

  • Stažení
  • Přidat do mých příruček
  • Tisk
  • Strana
    / 612
  • Tabulka s obsahem
  • ŘEŠENÍ PROBLÉMŮ
  • KNIHY
  • Hodnocené. / 5. Na základě hodnocení zákazníků
Zobrazit stránku 136
Preparing Samples
Voyager
Biospectrometry
Workstation User’s Guide 3-7
3
Sinapinic acid
Use sinapinic acid for peptides and proteins >10,000 Da.
Matrix concentration 10 mg/ml
Final sample concentration 0.1–5 pmol/µl
Solvents Acetonitrile, 0.1% TFA in deionized water
Preparation Follow the procedure in “Preparing matrix” on
page 3-5 and combine 700 µl of 0.1% TFA solution
in water, and 300 µl acetonitrile to 10 mg of solid
matrix.
If sample is contaminated with buffer, salt, or
detergent, instead of the proportions listed above,
combine 500 µl 0.1% TFA and 500 µl acetonitrile.
Air dry sample plate after loading sample and matrix.
NOTE: The organic concentration above assumes you are premixing sample
solution:matrix solution in a 1:10 dilution. If you are mixing sample solution:matrix
solution on the plate in a 1:1 dilution, increase the proportion of acetonitrile to
50 percent to prevent the matrix from precipitating. Adjust the other components
accordingly.
NOTE: A higher concentration of TFA (up to 3%) may improve sample solubility.
Crystals Uniform rhomboid shape (see Figure 3-1 on
page 3-34)
Stability Prepare daily.
Zobrazit stránku 136
1 2 ... 132 133 134 135 136 137 138 139 140 141 142 ... 611 612

Komentáře k této Příručce

Žádné komentáře